|
KMID : 0616120090400040298
|
|
Korean Journal of Pharmacognosy
2009 Volume.40 No. 4 p.298 ~ p.302
|
|
|
Simultaneous Quantification of Three Marker Compounds in Samultang by HPLC/DAD
|
|
Weon Jin-Bae
Ma Jin-Yeul
Lee Jae-Hoon
Ma Choong-Je
|
|
|
Abstract
|
|
|
Samultang is one of traditional medicine composed of Paeonia lactiflora, Angelica gigas, Rehmannia glutinosa and
Cnidium officinale. To develop simultaneous determination of paeoniflorin, decursin and 5-HMF in Samultang, a high performance liquid chromatography with diode array detector was used. To separate three marker components, Dionex C18 column (5 μm, 120 A, 4.6 mm× 150 mm) was used with a gradient elution system of water and methanol. UV wavelength of detector set at 230 nm and 280 nm. This method was validated by linearity, precision test and recovery test. Calibration curves of three standard components were showed good linear regression (R2>0.9973). LOD and LOQ ranged from 0.08μg/ml to 0.38 μg/ml and 0.25 μg/ml to 1.16 μg/ml, respectively. The relative standard deviations (RSDs) of data of the inter-day and intra-day experiments were less than 0.54% and 0.89%, respectively. The measured results of recovery test were varied from 93.36 to 107.79 with RSD values 0.01~1.45%. The established method was applied for separation of bio-conversion Samultang sample and compared with control sample.
|
|
|
KEYWORD
|
|
|
Samultang, simultaneous determination, HPLC/DAD, quantification
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|